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SCIENCE

The problem with liquid liposomes, (both injectable and oral delivery), is that they degrade quite quickly and have a short half-life. They are also more likely to break down in the stomach although it is not to say that they are ineffective, but less stable. Liquid liposomes tend to show low-solubility and sometimes leakage of the encapsulated molecules.

There have been some innovative methods invented to handle these problems and of these, so-called prolipsomes has been the most promising. Development of these dry liposomes began in the 1980’s for use in the pharmaceutical world.

LipoCellTech™ liposomes are in fact proliposomes

The stability of prolipsomes is far superior to regular liquid liposomes. They are a dry, free-flowing granular material (powder), that immediately forms a liposomal dispersion on contact with water or a biological fluid within the body. When the dry powders are hydrated with water or body fluids followed by gentle mixing, (as in the stomach), the contents rapidly disperse to give a liposomal suspension in the aqueous solution. In fact, THE LIPOSOMES FORMED, ONCE RECONSTRUCTED IN THE BODY, ARE SIMILAR TO CONVENTIONAL LIPOSOMES AND MORE STABLE AND UNIFORM IN SIZE.

To summarize; Our dry liposomes (proliposomes), are more stable, thus have a longer half-life. This way ensuring that a lot more of the ingredients in the liposomes reach the cells intact. 1, 2.

zoomed-in_photo_of_a_liposome

Photo of a single LipoCellTech™ powdered liposome after contact with body fluids. The phospholipids are on the outside and the ingredients in the middle. Magnified 30,000 X with an electron microscope. 

SCIENCE

The problem with liquid liposomes, (both injectable and oral delivery), is that they degrade quite quickly and have a short half-life. They are also more likely to break down in the stomach although it is not to say that they are ineffective, but less stable. Liquid liposomes tend to show low-solubility and sometimes leakage of the encapsulated molecules.

There have been some innovative methods invented to handle these problems and of these, so-called prolipsomes has been the most promising. Development of these dry liposomes began in the 1980’s for use in the pharmaceutical world.

LipoCellTech™ liposomes are in fact proliposomes

The stability of prolipsomes is far superior to regular liquid liposomes. They are a dry, free-flowing granular material (powder), that immediately forms a liposomal dispersion on contact with water or a biological fluid within the body. When the dry powders are hydrated with water or body fluids followed by gentle mixing, (as in the stomach), the contents rapidly disperse to give a liposomal suspension in the aqueous solution. In fact, THE LIPOSOMES FORMED, ONCE RECONSTRUCTED IN THE BODY, ARE SIMILAR TO CONVENTIONAL LIPOSOMES AND MORE STABLE AND UNIFORM IN SIZE.

zoomed-in_photo_of_a_liposome

Photo of a single LipoCellTech™ powdered liposome after contact with body fluids. The phospholipids are on the outside and the ingredients in the middle. Magnified 30,000 X with an electron microscope. 

To summarize; Our dry liposomes (proliposomes), are more stable, thus have a longer half-life. This way ensuring that a lot more of the ingredients in the liposomes reach the cells intact. 1, 2.

Studies of Lipsomal products

The quality of our LipoCellTech products is backed by science. Below you find various third-party human clinical studies on our LipoCellTech powders, that their show their superb effectiveness. Next to that you find the Verified Testing Results of several stability test, that show no degradation of the active ingredients after 3 years

Studies of LipoCellTech™ Lipsomal products

The quality of our LipoCellTech™ products is backed by science. Below you find various third-party human clinical studies on our LipoCellTech™ powders, that their show their superb effectiveness. Next to that you find the Verified Testing Results of several stability test, that show no degradation of the active ingredients after 3 years.

Study with

LipoCellTech™ vitamin B12

A single-dose, crossover study was conducted in six human subjects of equivalent doses of two dosage forms of vitamin B12 – a standard liposomal liquid and a liposomal powder-filled capsule (using LipoCellTech™ technology). Each subject was given 3 mg of LipoCellTech™ in one dosage form, followed by a washout period of 14 days, then dosing with the other dosage form.

All subjects (with one exception) had an increase above baseline at one or more time points over 135 minutes. Serum B12 increased six-fold on average after taking the LipoCellTech™ liposomal capsule, which was significantly more compared to the traditional liposomal liquid when comparing the highest peak of each subject’s serum B12.

study with
LipoCellTech™ vitamin b12

A single-dose, crossover study was conducted in six human subjects of equivalent doses of two dosage forms of vitamin B12 – a standard liposomal liquid and a liposomal powder-filled capsule (using LipoCellTech™ technology). Each subject was given 3 mg of LipoCellTech™ in one dosage form, followed by a washout period of 14 days, then dosing with the other dosage form.

All subjects (with one exception) had an increase above baseline at one or more time points over 135 minutes. Serum B12 increased six-fold on average after taking the LipoCellTech™ liposomal capsule, which was significantly more compared to the traditional liposomal liquid when comparing the highest peak of each subject’s serum B12.

Study with

LipoCellTech™ vitamin D

The Effect of a Liposomal Cholecalciferol Preparation on 25(OH)*

(Vitamin D Levels – A comparative, open-label study)

*25 OH is referring to the following: your body converts vitamin D to a chemical known as 25-hydroxyvitamin D, also called calcidiol. The 25-hydroxy vitamin D test is the best way to monitor vitamin D levels. The amount of 25-hydroxyvitamin D in your blood is a good indication of how much vitamin D your body has.

Abstract: Fifteen healthy participants underwent baseline 25(OH) Vitamin D testing, followed by supplementation with a liposomal product containing 125 mcg (5,000 IU) vitamin D and 400 mcg vitamin K (as MK7) daily for 30 days. Participants in this study had an average increase in serum 25(OH) Vitamin D of 73% over baseline. (Fig 1) For comparison, the average increase in 25(OH) Vitamin D with supplementation of a non-liposomal preparation for 30 days at this dosage is 16%.

Individuals who began the study with a 25(OH) Vitamin D level in the “insufficient” range (below 30 ng/mL) had an even greater response, averaging a 112% increase.

Conclusion: Supplementation with a liposomal vitamin D product for 30 days resulted in a much greater increase in serum 25(OH) Vitamin D than typical results after supplementation with a non-liposomal preparation (avg 73% vs 16%), proving the superior absorption of liposomal Vitamin D.

study with
LipoCellTech™ vitamin D

The Effect of a Liposomal Cholecalciferol Preparation on 25(OH)*

(Vitamin D Levels – A comparative, open-label study)

*25 OH is referring to the following: your body converts vitamin D to a chemical known as 25-hydroxyvitamin D, also called calcidiol. The 25-hydroxy vitamin D test is the best way to monitor vitamin D levels. The amount of 25-hydroxyvitamin D in your blood is a good indication of how much vitamin D your body has.

Abstract: Fifteen healthy participants underwent baseline 25(OH) Vitamin D testing, followed by supplementation with a liposomal product containing 125 mcg (5,000 IU) vitamin D and 400 mcg vitamin K (as MK7) daily for 30 days. Participants in this study had an average increase in serum 25(OH) Vitamin D of 73% over baseline. (Fig 1) For comparison, the average increase in 25(OH) Vitamin D with supplementation of a non-liposomal preparation for 30 days at this dosage is 16%.

Individuals who began the study with a 25(OH) Vitamin D level in the “insufficient” range (below 30 ng/mL) had an even greater response, averaging a 112% increase.

Conclusion: Supplementation with a liposomal vitamin D product for 30 days resulted in a much greater increase in serum 25(OH) Vitamin D than typical results after supplementation with a non-liposomal preparation (avg 73% vs 16%), proving the superior absorption of liposomal Vitamin D.

Study with LipoCellTech™ Vitamin C

PART 1: How much LipoCellTech™ Vitamin C absorbs into a person’s blood (bioavailability)?

While high doses of regular vitamin C powder or capsules will be rejected at high rates from your body, LipoCellTech™ Vitamin C is a fantastic alternative.

Figure 1 shows that, compared with “normal” Vitamin C ingestion (single dose of 4 g), ingestion of LipoCellTech™ Vitamin C (also 4 g) leads to appreciably greater circulating Vitamin C concentrations.  Hence, liposomes facilitate Vitamin C delivery to the blood.

PART 2: What will Vitamin C do once in the blood (efficacy)?

The next step in the study is to determine if the Vitamin C concentration is meaningful.  In this regard, an ischemia reperfusion study was performed.  Ischemia describes any tissue that has been deprived of blood and oxygen.

Reperfusion occurs when the blood and oxygen supply is returned.  Unfortunately, reperfusion can cause massive oxidative stress on your cardiovascular system, as the oxygen interacts with metabolites produced by the oxygen-deprived tissues.  Per the blue line in the diagram here, oxidative stress was reduced even during the increased oxidative stress of reperfusion. 3

study with
LipoCellTech™ vitamin C

PART 1: How much LipoCellTech™ Vitamin C absorbs into a person’s blood (bioavailability)?

While high doses of regular vitamin C powder or capsules will be rejected at high rates from your body, LipoCellTech™ Vitamin C is a fantastic alternative.

Figure 1 shows that, compared with “normal” Vitamin C ingestion (single dose of 4 g), ingestion of LipoCellTech™ Vitamin C (also 4 g) leads to appreciably greater circulating Vitamin C concentrations.  Hence, liposomes facilitate Vitamin C delivery to the blood.

PART 2: What will Vitamin C do once in the blood (efficacy)?

The next step in the study is to determine if the Vitamin C concentration is meaningful.  In this regard, an ischemia reperfusion study was performed.  Ischemia describes any tissue that has been deprived of blood and oxygen.

Reperfusion occurs when the blood and oxygen supply is returned.  Unfortunately, reperfusion can cause massive oxidative stress on your cardiovascular system, as the oxygen interacts with metabolites produced by the oxygen-deprived tissues.  Per the blue line in the diagram here, oxidative stress was reduced even during the increased oxidative stress of reperfusion. 3

Study with LipoCellTech™ Glutathione

Study shows that oral supplementation with liposomal glutathione elevates body stores of glutathione and markers of immune function

Abstract (from Pub Med)

Background/objectives: Glutathione (GSH) is the most abundant endogenous antioxidant and a critical regulator of oxidative stress. Maintenance of optimal tissues for GSH levels may be an important strategy for the prevention of oxidative stress-related diseases. We investigated if oral administration of liposomal GSH is effective at enhancing GSH levels in vivo.

Subjects/methods: A 1-month pilot clinical study of oral liposomal GSH administration at two doses (500 and 1000 mg of GSH per day) was conducted in healthy adults. GSH levels in whole blood, erythrocytes, plasma and peripheral blood mononuclear cells (PBMCs)* were assessed in 12 subjects at the baseline and after 1, 2 and 4 weeks of GSH administration.

* PBMC stands for peripheral blood mononuclear cells. PBMCs are a variety of specialized immune cells that work together to protect our bodies from harmful pathogens. Their location in peripheral blood is significant because they act as a line of defense from infection and disease.

Results:
 GSH levels were elevated after 1 week with maximum increases of 40% in whole blood, 25% in erythrocytes, 28% in plasma and 100% in PBMCs occurring after 2 weeks (P<0.05). GSH increases were accompanied by reductions in oxidative stress biomarkers, including decreases of 35% in plasma 8-isoprostane and 20% in oxidized:reduced GSH ratios (P<0.05). Enhancements in immune function markers were observed with liposomal GSH administration including Natural killer (NK) cell cytotoxicity, which was elevated by up to 400% by 2 weeks (P<0.05), and lymphocyte proliferation, which was elevated by up to 60% after 2 weeks (P<0.05). Overall, there were no differences observed between dose groups, but statistical power was limited due to the small sample size in this study.

Conclusions: Collectively, these preliminary findings support the effectiveness of daily liposomal GSH administration at elevating stores of GSH and impacting the immune function and levels of oxidative stress.

study with
LipoCellTech™ Glutathione

Study shows that oral supplementation with liposomal glutathione elevates body stores of glutathione and markers of immune function

Abstract (from Pub Med)

Background/objectives: Glutathione (GSH) is the most abundant endogenous antioxidant and a critical regulator of oxidative stress. Maintenance of optimal tissues for GSH levels may be an important strategy for the prevention of oxidative stress-related diseases. We investigated if oral administration of liposomal GSH is effective at enhancing GSH levels in vivo.

Subjects/methods: A 1-month pilot clinical study of oral liposomal GSH administration at two doses (500 and 1000 mg of GSH per day) was conducted in healthy adults. GSH levels in whole blood, erythrocytes, plasma and peripheral blood mononuclear cells (PBMCs)* were assessed in 12 subjects at the baseline and after 1, 2 and 4 weeks of GSH administration.4

* PBMC stands for peripheral blood mononuclear cells. PBMCs are a variety of specialized immune cells that work together to protect our bodies from harmful pathogens. Their location in peripheral blood is significant because they act as a line of defense from infection and disease.

Results: GSH levels were elevated after 1 week with maximum increases of 40% in whole blood, 25% in erythrocytes, 28% in plasma and 100% in PBMCs occurring after 2 weeks (P<0.05). GSH increases were accompanied by reductions in oxidative stress biomarkers, including decreases of 35% in plasma 8-isoprostane and 20% in oxidized:reduced GSH ratios (P<0.05). Enhancements in immune function markers were observed with liposomal GSH administration including Natural killer (NK) cell cytotoxicity, which was elevated by up to 400% by 2 weeks (P<0.05), and lymphocyte proliferation, which was elevated by up to 60% after 2 weeks (P<0.05). Overall, there were no differences observed between dose groups, but statistical power was limited due to the small sample size in this study.

Conclusions: Collectively, these preliminary findings support the effectiveness of daily liposomal GSH administration at elevating stores of GSH and impacting the immune function and levels of oxidative stress.

STABILITY TEST:
Verified Testing Results

We are proud to present the verified test results of the active ingredient content of our supplement over the past three years.

These results are a confirmation of what we always foresaw to be true: that our supplement preserved a reliable source of the active ingredient. Independent studies have confirmed the consistency and quality of the active ingredients, reinforcing our confidence in their effectiveness. Our test results were obtained through various measurement methods, which collectively provide a comprehensive and accurate picture of the active ingredient content in our supplement.

Variations in percentages over the years are natural and may be the result of various factors. However, through our commitment to high-quality raw materials, careful manufacturing processes and rigorous quality controls, we continue to pursue our mission to provide consumers with a supplement that meets high quality in a reliable and consistent manner.

STABILITY TESTS:
verified testing results

We are proud to present the verified test results of the active ingredient content of our supplement over the past three years.

These results are a confirmation of what we always foresaw to be true: that our supplement preserved a reliable source of the active ingredient. Independent studies have confirmed the consistency and quality of the active ingredients, reinforcing our confidence in their effectiveness. Our test results were obtained through various measurement methods, which collectively provide a comprehensive and accurate picture of the active ingredient content in our supplement.

Variations in percentages over the years are natural and may be the result of various factors. However, through our commitment to high-quality raw materials, careful manufacturing processes and rigorous quality controls, we continue to pursue our mission to provide consumers with a supplement that meets high quality in a reliable and consistent manner.

Interested in more?

Are you interested in more in-depth studies? Our experts are ready for you. If you contact us via the contact form below, we can share more studies and data with you!

References

Akbarzadeh A, Rezaei-Sadabady R, Davaran S, et al. Liposome: classification, preparation, and applications. Nanoscale Res Lett. 2013;8(1):102. Published 2013 Feb 22. doi:10.1186/1556-276X-8-102.

2. Singh, Namita & Kushwaha, Poonam & Ahmad, Usama & Abdullah, Mohammad. (2019). Proliposomes: An Approach for the Development of Stable Liposome. Ars Pharmaceutica. 60. 231-240. 10.30827/ars.v60i4.8517.

3. Davis, J. L., Paris, H. L., Beals, J. W., Binns, S. E., Giordano, G. R., Scalzo, R. L., Schweder, M. M., Blair, E., & Bell, C. (2016). Liposomal-encapsulated Ascorbic Acid: Influence on Vitamin C Bioavailability and Capacity to Protect Against Ischemia-Reperfusion Injury. Nutrition and metabolic insights9, 25–30. https://doi.org/10.4137/NMI.S39764.

4. Sinha R, Sinha I, Calcagnotto A, Trushin N, Haley JS, Schell TD, Richie JP Jr. Oral supplementation with liposomal glutathione elevates body stores of glutathione and markers of immune function. Eur J Clin Nutr. 2018 Jan;72(1):105-111. doi: 10.1038/ejcn.2017.132. Epub 2017 Aug 30. PMID: 28853742; PMCID: PMC6389332.

references

Akbarzadeh A, Rezaei-Sadabady R, Davaran S, et al. Liposome: classification, preparation, and applications. Nanoscale Res Lett. 2013;8(1):102. Published 2013 Feb 22. doi:10.1186/1556-276X-8-102.

2. Singh, Namita & Kushwaha, Poonam & Ahmad, Usama & Abdullah, Mohammad. (2019). Proliposomes: An Approach for the Development of Stable Liposome. Ars Pharmaceutica. 60. 231-240. 10.30827/ars.v60i4.8517.

3. Davis, J. L., Paris, H. L., Beals, J. W., Binns, S. E., Giordano, G. R., Scalzo, R. L., Schweder, M. M., Blair, E., & Bell, C. (2016). Liposomal-encapsulated Ascorbic Acid: Influence on Vitamin C Bioavailability and Capacity to Protect Against Ischemia-Reperfusion Injury. Nutrition and metabolic insights9, 25–30. https://doi.org/10.4137/NMI.S39764.

4. Sinha R, Sinha I, Calcagnotto A, Trushin N, Haley JS, Schell TD, Richie JP Jr. Oral supplementation with liposomal glutathione elevates body stores of glutathione and markers of immune function. Eur J Clin Nutr. 2018 Jan;72(1):105-111. doi: 10.1038/ejcn.2017.132. Epub 2017 Aug 30. PMID: 28853742; PMCID: PMC6389332.

Get in touch
with us today

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LipoCellTech™ Head office

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535 Rogers Street • Clearwater, FL 33756 • RH10 4HQ • United States

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